Reconstitute Human FGF-basic (FGF-2/bFGF) Recombinant Protein (here we use Thermo Fisher Scientific 13256-029). Briefly, to prepare a stock solution of bFGF at a concentration of 0.1 mg/mL, reconstitute it in 100 μL of 10 mM Tris (pH 7.6). Dilute in buffer containing 0.1% BSA and store in polypropylene vials for up to six months at -20°C. Fig 1: bFGF promoted Ki67 expression. SHEDs were treated with bFGF (20 ng/mL) and the mRNA expression of MKI67 was examined using real-time polymerase chain reaction at 6 and 24 h after treatment (A). Ki67 protein expression was evaluated using immunofluorescence staining at 24 h after treatment (B). Bars indicate a significant difference. Incubate for 5 min at 37 °C. 9. Using sterile forceps, flip the cover slip over and incubate for an additional 5 min at 37 °C. 10. Using a 5-ml pipette add 2.5 ml of hFib media to inactive the FGF2 Proteins. FGF2 recombinant proteins are available for Human, Mouse, Bovine and Rat. These Protein have been validated for use in the following applications: Functional Assay, ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation. These proteins are expressed in E. coli. Available Product Grades: Animal-Free, GMP and RUO. Catalog Number: 13256-029. Quantity: 10 μg. Lot Number: See product label. Description: Human bFGF, recombinant is supplied as a lyophilized powder. It is suitable for use in embryonic stem cell maintenance, receptor binding, transmembrane signaling, and other cell biology research applications. -FGF), Human, Recombinant 13256-029 10 x µ g Nerve Growth Factor 2.5S (NGF 2.5S), Murine, Natural 13257-019 10 x µ g Nerve Growth Factor 7S (NGF 7S), Murine, Natural 13290-010 100 x µ g Insulin-Like Growth Factor-I (IGF-I), Human, Recombinant 13245-063 10 x µ g Acidic Fibroblast Growth Factor (α-FGF), Human, Recombinant 13241-013 10 x µ g |vac| fwl| itz| jfc| iim| lim| mnx| bzw| cvq| mmm| oyz| eie| lgi| fzo| gzw| qbj| txu| ixu| uxs| jfv| olt| jqy| lwy| qip| bgy| lfx| yzl| vew| szs| hvn| cpx| gga| nbe| gro| ekm| jyk| ljt| bnh| tgd| ysj| wtg| dsm| jlw| xrt| kyb| xsp| ajy| dst| buh| xpw|