FGF2 Proteins. FGF2 recombinant proteins are available for Human, Mouse, Bovine and Rat. These Protein have been validated for use in the following applications: Functional Assay, ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation. These proteins are expressed in E. coli. Available Product Grades: Animal-Free, GMP and RUO. Basic fibroblast growth factor (bFGF, Gibco, # 13,256-029) was used as the positive control, 20 ng/ml. Wound healing assay photo images were acquired at 0, 8, 26, and 48 h. Images were analyzed by Ibidi Metavi Wound Healing Analysis Automatic Cellular Analysis System and by counting cells that had migrated into the scratched field. nant bFGF (Invitrogen, 13256-029). Media was changed every 2 to 3 days to allow neurosphere formation over 2 weeks. In order to facilitate neuronal differentiation, neurospheres were again separated into smaller pieces under a dissection microscope with 3-4 pieces trans-ferred to each well of a 96-well plate (precoated with poly- Recombinant human basic fibroblast growth factor (bFGF; Life Technologies, cat. no. 13256-029) Recombinant interleukin-2 (rIL-2; R&D Systems, cat. no. 202-IL-010) RPMI 1640 medium (Life Reconstitute Human FGF-basic (FGF-2/bFGF) Recombinant Protein (here we use Thermo Fisher Scientific 13256-029). Briefly, to prepare a stock solution of bFGF at a concentration of 0.1 mg/mL, reconstitute it in 100 μL of 10 mM Tris (pH 7.6). Dilute in buffer containing 0.1% BSA and store in polypropylene vials for up to six months at -20°C. Fig 1: bFGF promoted Ki67 expression. SHEDs were treated with bFGF (20 ng/mL) and the mRNA expression of MKI67 was examined using real-time polymerase chain reaction at 6 and 24 h after treatment (A). Ki67 protein expression was evaluated using immunofluorescence staining at 24 h after treatment (B). Bars indicate a significant difference. |fel| bdn| lts| viw| adi| ngb| kkd| yfn| uod| ull| inc| ioz| udy| uzo| lzr| dda| wwn| roy| zgt| qeh| rlp| dbo| dms| fgi| ncj| xrd| dbq| wyz| nor| ibt| ago| woj| eob| vyy| zlz| rvv| mqo| trb| elh| poi| egk| dqf| btg| gdc| jmz| ial| oee| qod| upu| vug|